Figure 1. Western blot analysis of C20orf74/RALGAPA2 using anti-C20orf74/RALGAPA2 antibody (A10416-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C20orf74/RALGAPA2 antigen affinity purified polyclonal antibody (Catalog # A10416-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C20orf74/RALGAPA2 at approximately 250 kDa. The expected band size for C20orf74/RALGAPA2 is at 211 kDa.
Figure 1. Western blot analysis of C20orf74/RALGAPA2 using anti-C20orf74/RALGAPA2 antibody (A10416-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C20orf74/RALGAPA2 antigen affinity purified polyclonal antibody (Catalog # A10416-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C20orf74/RALGAPA2 at approximately 250 kDa. The expected band size for C20orf74/RALGAPA2 is at 211 kDa.
Anti-C20orf74/RALGAPA2 Antibody Picoband(r)
A10416-1-FITC
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetRALGAPA2
Overview
- SupplierBoster Bio
- Product NameAnti-C20orf74/RALGAPA2 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateFITC
- Gene ID57186
- Target nameRALGAPA2
- Target descriptionRal GTPase activating protein catalytic subunit alpha 2
- Target synonyms250 kDa substrate of Akt; akt substrate AS250; AS250; bA287B20.1; C20orf74; dJ1049G11; dJ1049G11.4; p220; Ral GTPase activating protein catalytic alpha subunit 2; Ral GTPase activating protein, alpha subunit 2 (catalytic); ral GTPase-activating protein alpha subunit 2; ral GTPase-activating protein subunit alpha-2; RapGAPalpha2
- HostRabbit
- IsotypeIgG
- Protein IDQ2PPJ7
- Protein NameRal GTPase-activating protein subunit alpha-2
- Scientific DescriptionBoster Bio Anti-C20orf74/RALGAPA2 Antibody Picoband® catalog # A10416-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203