IHC analysis of Calbindin using anti-Calbindin antibody (PB9045). Calbindin was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calbindin Antibody (PB9045) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of Calbindin using anti-Calbindin antibody (PB9045). Calbindin was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calbindin Antibody (PB9045) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Calbindin/CALB1 Antibody Picoband(r)
PB9045
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Equine, Human, Mouse, Rat
TargetCALB1
Overview
- SupplierBoster Bio
- Product NameAnti-Calbindin Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for Calbindin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- Applications SupplierIHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID793
- Target nameCALB1
- Target descriptioncalbindin 1
- Target synonymsCALB; calbindin; calbindin 1, (28kD); calbindin 1, 28kDa; calbindin 27; calbindin D28; D-28K; RTVL-H protein; vitamin D-dependent calcium-binding protein, avian-type
- HostRabbit
- IsotypeIgG
- Protein IDP05937
- Protein NameCalbindin
- Scientific DescriptionBoster Bio Anti-Calbindin/CALB1 Antibody Picoband® catalog # PB9045. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Equine, Human, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat, Bovine, Horse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203