Immunocytochemistry: Rat mixed neuron / glial cultures stained with ARG10703 anti-Calretinin antibody [6A9] (red) at 1:2000, and co-stained with chicken polyclonal antibody to Vimentin (green). Calretinin is prominently expressed in small number of interneurons, while astrocytes and fibroblasts were visualized with the vimentin antibody.
![Immunohistochemistry: Frozen sections of adult Rat cortical (45 microM; fixed by transcardial perfusion with 4% paraformaldehyde) was stained with ARG10703 anti-Calretinin antibody [6A9] at 1:1000 (red) and co-stained with chicken polyclonal antibody to calbinidin (green). In the motor cortex, calretinin is expressed in a small population of interneurons that do not express calbindin. Because each antibody specifically labels a different population of cells exclusively, the cells are either stained with red, or green.](https://www.arigobio.com/files/product/12496/ARG10703_IHC_1.jpg "Immunohistochemistry: Frozen sections of adult Rat cortical (45 microM; fixed by transcardial perfusion with 4% paraformaldehyde) was stained with ARG10703 anti-Calretinin antibody [6A9] at 1:1000 (red) and co-stained with chicken polyclonal antibody to calbinidin (green). In the motor cortex, calretinin is expressed in a small population of interneurons that do not express calbindin. Because each antibody specifically labels a different population of cells exclusively, the cells are either stained with red, or green.")
![Immunohistochemistry: Frozen sections of adult Mouse brain hippocampal (45 microM; fixed by transcardial perfusion with 4% paraformaldehyde) was stained with ARG10703 anti-Calretinin antibody [6A9] at 1:1000 (red) and co-stained with antibody to calbinidin (green). In the stratum radiatum of CA1 region, calretinin expresses in a small number of interneurons that do not express calbindin. As a result, our antibodies label different neurons in either red or green. Insets are high-magnification images of the boxed area in each picture. Blue is a hoechst DNA staining.](https://www.arigobio.com/files/product/12496/ARG10703_IHC_2.jpg "Immunohistochemistry: Frozen sections of adult Mouse brain hippocampal (45 microM; fixed by transcardial perfusion with 4% paraformaldehyde) was stained with ARG10703 anti-Calretinin antibody [6A9] at 1:1000 (red) and co-stained with antibody to calbinidin (green). In the stratum radiatum of CA1 region, calretinin expresses in a small number of interneurons that do not express calbindin. As a result, our antibodies label different neurons in either red or green. Insets are high-magnification images of the boxed area in each picture. Blue is a hoechst DNA staining.")
![Immunohistochemistry: Frozen section of Rat hippocampus stained with ARG10703 anti-Calretinin antibody [6A9] (green) at 1:2000 dilution and costained with ARG10686 anti-Parvalbumin antibody (red) at 1:1000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 microM, and free-floating sections were stained with above antibodies.)](https://www.arigobio.com/files/product/12496/ARG10703_IHC-Fr_190729.jpg "Immunohistochemistry: Frozen section of Rat hippocampus stained with ARG10703 anti-Calretinin antibody [6A9] (green) at 1:2000 dilution and costained with ARG10686 anti-Parvalbumin antibody (red) at 1:1000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 microM, and free-floating sections were stained with above antibodies.)")
![Western blot: Rat brain, pavalbumin, calretinin, and calbindin recombinant proteins was stained with ARG10703 anti-Calretinin antibody [6A9] at 1:5000 dilution.](https://www.arigobio.com/files/product/12496/ARG10703_WB_171108.jpg "Western blot: Rat brain, pavalbumin, calretinin, and calbindin recombinant proteins was stained with ARG10703 anti-Calretinin antibody [6A9] at 1:5000 dilution.")
![Western blot: Rat brain, Rat spinal cord, Mouse brain, Mouse spinal cord and Cow spinal cord lysates stained with ARG10703 anti-Calretinin antibody [6A9] (green) at 1:2000 dilution.](https://www.arigobio.com/files/product/12496/ARG10703_WB_190729.jpg "Western blot: Rat brain, Rat spinal cord, Mouse brain, Mouse spinal cord and Cow spinal cord lysates stained with ARG10703 anti-Calretinin antibody [6A9] (green) at 1:2000 dilution.")
![Western blot: 15 microg of Rat brain lysate stained with ARG10703 anti-Calretinin antibody [6A9] at 1:2500 dilution.](https://www.arigobio.com/files/product/12496/ARG10703_WB_Rev_RatBrain_170714.jpg "Western blot: 15 microg of Rat brain lysate stained with ARG10703 anti-Calretinin antibody [6A9] at 1:2500 dilution.")
Immunocytochemistry: Rat mixed neuron / glial cultures stained with ARG10703 anti-Calretinin antibody [6A9] (red) at 1:2000, and co-stained with chicken polyclonal antibody to Vimentin (green). Calretinin is prominently expressed in small number of interneurons, while astrocytes and fibroblasts were visualized with the vimentin antibody.
anti-Calretinin antibody [6A9]
ARG10703
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman
TargetCALB2
Overview
- SupplierArigo Biolaboratories
- Product Nameanti-Calretinin antibody [6A9]
- Delivery Days Customer23
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID6A9
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID794
- Target nameCALB2
- Target descriptioncalbindin 2
- Target synonyms29 kDa calbindin; CAB29; CAL2; calbindin 2, (29kD, calretinin); calbindin D29K; calretinin; CR; testicular secretory protein Li 8
- HostMouse
- IsotypeIgA
- Scientific DescriptionThis gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203