Anti-Capsid protein VP1 [MabB], Rabbit IgG, kappa

Absolute Antibody

Anti-Capsid protein VP1 [MabB], Rabbit IgG, kappa

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This antibody is specific for CPV-2s, FPVs capsid (site A). It also cross-reacts with MEV and RPV.

The original version of this antibody (rat IgG2b) was raised and tested against CPA, FPV, and MEV and was found to inhibit and neutralize CPV, FPV, and MEV-a and -b in neutralization and HI assays. It was also used in RIAs. In the HI test, it was found to inhibit CPV-a;-b;-c-;-d; and -e, FPV-a; -b; -c, RPV-a; -b; -c; -d; and -e, and MEV-a; -b; -c; -d; -e; -f; -g; and -h. In the RIA, it was found to react with CPV-d and FPV-b (Parrish and Carmichael, 1983; PMID: 6194613). The Fab version of this antibody was used in a cryoEM analysis of capsid:Fab complexes, and its Kd was estimated via ELISA as ~6.1 x 10-9 M (Nelson et al., 2007; PMID: 17217977). The Fab version of this antibody was crystallized, and its structure was determined (Hafenstein et al., 2009; PMID: 19321620).

ELISA, Neutralisation/Blocking, Other Application, RadioImmunoAssay

Research Use Only

Monoclonal

MabB

Rabbit

IgG

Virus

-20°C,2°C to 8°C

12352203