Figure 2. Flow Cytometry analysis of Hela cells using anti-CPM antibody (A01650). Overlay histogram showing Hela cells stained with A01650 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CPM Antibody (A01650, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 2. Flow Cytometry analysis of Hela cells using anti-CPM antibody (A01650). Overlay histogram showing Hela cells stained with A01650 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CPM Antibody (A01650, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Anti-Carboxypeptidase M/CPM Antibody Picoband(r)
A01650-CY3
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman
TargetCPM
Overview
- SupplierBoster Bio
- Product NameAnti-Carboxypeptidase M/CPM Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateCy3
- Gene ID1368
- Target nameCPM
- Target descriptioncarboxypeptidase M
- Target synonymscarboxypeptidase M; renal carboxypeptidase; urinary carboxypeptidase B
- HostRabbit
- IsotypeIgG
- Protein IDP14384
- Protein NameCarboxypeptidase M
- Scientific DescriptionBoster Bio Anti-Carboxypeptidase M/CPM Antibody Picoband® catalog # A01650. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203