Immunohistochemical analysis of paraffin-embedded human tonsil, using Caspase-3 Antibody(M00334-5) CASP3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP3 Antibody (M00334-5)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human tonsil, using Caspase-3 Antibody(M00334-5) CASP3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP3 Antibody (M00334-5)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody
M00334-5
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCASP3
Overview
- SupplierBoster Bio
- Product NameAnti-Caspase-3 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDAOE-3
- FormulationLiquid
- Gene ID836
- Target nameCASP3
- Target descriptioncaspase 3
- Target synonymsapopain; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; caspase-3; CPP32; CPP-32; CPP32B; cysteine protease CPP32; PARP cleavage protease; procaspase3; protein Yama; SCA-1; SREBP cleavage activity 1
- HostRabbit
- IsotypeIgG
- Protein IDP42574
- Protein NameCaspase-3
- Scientific DescriptionBoster Bio Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody catalog # M00334-5. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203
References
- Sonodynamic therapy in atherosclerosis by curcumin nanosuspensions: Preparation design, efficacy evaluation, and mechanisms analysis. Jiang L et al., 2020 Jan, Eur J Pharm BiopharmRead more
- MiR-429 regulates rat liver regeneration and hepatocyte proliferation by targeting JUN/MYC/BCL2/CCND1 signaling pathway. Zhang C et al., 2018 Oct, Cell SignalRead more