Figure 1. Western blot analysis of Caspase-6(P18) using anti-Caspase-6(P18) antibody (PA1441). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-6(P18) antigen affinity purified polyclonal antibody (Catalog # PA1441) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-6(P18) at approximately 35 kDa. The expected band size for Caspase-6(P18) is at 35 kDa.
Figure 1. Western blot analysis of Caspase-6(P18) using anti-Caspase-6(P18) antibody (PA1441). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-6(P18) antigen affinity purified polyclonal antibody (Catalog # PA1441) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-6(P18) at approximately 35 kDa. The expected band size for Caspase-6(P18) is at 35 kDa.
Anti-Caspase-6(P18)/CASP6 Antibody Picoband(r)
PA1441
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityEquine, Human
TargetCASP6
Overview
- SupplierBoster Bio
- Product NameAnti-Caspase-6(P18) Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
- Applications SupplierIHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID839
- Target nameCASP6
- Target descriptioncaspase 6
- Target synonymsapoptotic protease MCH-2; caspase 6, apoptosis-related cysteine peptidase; caspase 6, apoptosis-related cysteine protease; caspase-6; MCH2
- HostRabbit
- IsotypeIgG
- Protein IDP55212
- Protein NameCaspase-6
- Scientific DescriptionBoster Bio Anti-Caspase-6 (P18)/CASP6 Antibody catalog # PA1441. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityEquine, Human
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203