Immunohistochemical analysis of paraffin-embedded human liver cancer, using Caspase-9 Antibody(M00080-3) CASP9 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP9 Antibody (M00080-3)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human liver cancer, using Caspase-9 Antibody(M00080-3) CASP9 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP9 Antibody (M00080-3)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody
M00080-3
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCASP9
Overview
- SupplierBoster Bio
- Product NameAnti-Caspase-9 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDAOC-3
- FormulationLiquid
- Gene ID842
- Target nameCASP9
- Target descriptioncaspase 9
- Target synonymsAPAF3; APAF-3; apoptotic protease activating factor 3; apoptotic protease MCH-6; caspase 9, apoptosis-related cysteine peptidase; caspase-9; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; PPP1R56; protein phosphatase 1, regulatory subunit 56
- HostRabbit
- IsotypeIgG
- Protein IDP55211
- Protein NameCaspase-9
- Scientific DescriptionBoster Bio Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody catalog # M00080-3. Tested in WB, IHC, ICC/IF, Flow Cytometry, IP applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203