Immunohistochemical analysis on paraffin-embedded tisf paraffin-embedded human ovarian cancer tissue, using Cathepsin D Antibody(M01361) CTSD was detected isue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTSD Antibody (M01361)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis on paraffin-embedded tisf paraffin-embedded human ovarian cancer tissue, using Cathepsin D Antibody(M01361) CTSD was detected isue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTSD Antibody (M01361)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Cathepsin D CTSD Rabbit Monoclonal Antibody
M01361
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCTSD
Overview
- SupplierBoster Bio
- Product NameAnti-Cathepsin D Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDCEF-3
- FormulationLiquid
- Gene ID1509
- Target nameCTSD
- Target descriptioncathepsin D
- Target synonymscathepsin D; ceroid-lipofuscinosis, neuronal 10; CLN10; CPSD; epididymis secretory sperm binding protein Li 130P; HEL-S-130P; lysosomal aspartyl peptidase; lysosomal aspartyl protease
- HostRabbit
- IsotypeIgG
- Protein IDP07339
- Protein NameCathepsin D
- Scientific DescriptionBoster Bio Anti-Cathepsin D CTSD Rabbit Monoclonal Antibody catalog # M01361. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Identification of chemoresistance-related cell-surface glycoproteins in leukemia cells and functional validation of candidate glycoproteins. Sun Z et al., 2014 Mar 7, J Proteome ResRead more