Figure 1. IHC analysis of Cathepsin D using anti-Cathepsin D antibody (PB9047). Cathepsin D was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Cathepsin D Antibody (PB9047) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of Cathepsin D using anti-Cathepsin D antibody (PB9047). Cathepsin D was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Cathepsin D Antibody (PB9047) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-Cathepsin D/CTSD Antibody Picoband(r)
PB9047-CY3
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCTSD
Overview
- SupplierBoster Bio
- Product NameAnti-Cathepsin D/CTSD Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for Cathepsin D is approximately 5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateCy3
- Gene ID1509
- Target nameCTSD
- Target descriptioncathepsin D
- Target synonymscathepsin D; ceroid-lipofuscinosis, neuronal 10; CLN10; CPSD; epididymis secretory sperm binding protein Li 130P; HEL-S-130P; lysosomal aspartyl peptidase; lysosomal aspartyl protease
- HostRabbit
- IsotypeIgG
- Protein IDP07339
- Protein NameCathepsin D
- Scientific DescriptionBoster Bio Anti-Cathepsin D/CTSD Antibody Picoband® catalog # PB9047. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203