
Figure 1. Western blot analysis of CBS using anti-CBS antibody (RP1041). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat pancrease tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse pancrease tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBS antigen affinity purified polyclonal antibody (Catalog # RP1041) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBS at approximately 65 kDa. The expected band size for CBS is at 61 kDa.
Anti-Cystathionine beta-synthase CBS Antibody Picoband(r)

RP1041
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCBS
Overview
- SupplierBoster Bio
- Product NameAnti-CBS Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for CBS is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID875
- Target nameCBS
- Target descriptioncystathionine beta-synthase
- Target synonymsbeta-thionase; CBSL; cystathionine beta-synthase; Cystathionine beta-synthase-like protein; HIP4; methylcysteine synthase; serine sulfhydrase
- HostRabbit
- IsotypeIgG
- Protein IDP35520
- Protein NameCystathionine beta-synthase
- Scientific DescriptionBoster Bio Anti-Cystathionine beta-synthase CBS Antibody catalog # RP1041. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203