Anti-CCL11 [CAT-213 (Bertilimumab; iCo-008)]

Absolute Antibody

Anti-CCL11 [CAT-213 (Bertilimumab; iCo-008)]

9

This antibody binds human eotaxin (CCL11). This CC chemokine directly promotes the accumulation of eosinophils in response to the presence of allergens, which is a prominent feature of allergic inflammatory reactions. It binds to CCR3.

The binding specificity of this antibody for eotaxin 1 was demonstrated using ELISA. The scFv version of this antibody binds human eotaxin1 with a dissociation constant (KD) of 80.4 pM. This antibody demonstrated inhibition of eotaxin1 stimulated chemotaxis of L1.2-hCCR3 cells in vitro. This antibody also inhibited eotaxin1-induced eosinophil shape change with an IC50 of 0.71 nM. In vivo administration of human eotaxin1 to the air pouch of ovalbumin-sensitized mice treated with IL-5 caused a significant dose-related increase in the recruitment of leukocytes to the pouch after 6 h (PMID: 16973884). A paper studied the contribution of eotaxin to chemotactic activity was studied by using a high-affinity neutralizing human anti-eotaxin antibody, CAT213. It was reported that this antibody significantly inhibited chemotactic activity of eosinophils in all asthmatic groups (PMID: 15001461)

ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

CAT-213 (Bertilimumab; iCo-008)

CCL11

C-C motif chemokine ligand 11

chemokine (C-C motif) ligand 11; eosinophil chemotactic protein; eotaxin; eotaxin-1; SCYA11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Mouse

IgG2b

Eotaxin

This is a reformatted mouse IgG2b Fc Silent™ antibody, based on the original mouse IgG4 format, created for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203