Anti-CCL2 [CNTO 888 (Carlumab)]

Absolute Antibody

Anti-CCL2 [CNTO 888 (Carlumab)]

7

This antibody specifically binds human CCL2 and does not cross react with mouse CCL2 or other human CC chemokines. It is reported that CNTO 888 recognizes a conformational epitope encompassing residues 18-24 and 45-51 that overlaps the mapped receptor bin

CCL2 is implicated in the pathogenesis of certain inflammatory diseases and cancer. Binding of CCL2 to its receptor CCR2 triggers calcium mobilization and chemotaxis. CNTO 888 is a neutralizing anti-CCL2 antibody that binds human CCL2 with high affinity of Kd=22pM and inhibits CCL2 binding to its receptor. The systemic administration of anti-CCL2 neutralizing antibodies (CNTO888 and C1142) significantly retarded tumor growth and attenuated CD68+ macrophage infiltration, which was accompanied by a significant decrease in microvascular density (PMID: 17710158). Phase 1 trials in 44 patients with solid tumors concluded that CNTO888 was well tolerated in individuals with evidence of transient free CCL2 suppression and preliminary antitumor activity (PMID: 23385782). This antibody was also capable of inhibiting growth of breast cancer when administered individually or as a cocktail (Campion et al, 2009). A phase 2 study in 46 patients with castration-resistant prostate cancer (CRPC) concluded that carlumab was well-tolerated but did not block the CCL2/CCR2 axis or show antitumor activity as a single agent in metastatic CRPC (PMID: 22907596).

Neutralisation/Blocking

Inhibit; Block; Therapeutic

Research Use Only

Monoclonal

CNTO 888 (Carlumab)

CCL2

C-C motif chemokine ligand 2

C-C motif chemokine 2; chemokine (C-C motif) ligand 2; GDCF-2; HC11; HSMCR30; MCAF; MCP1; MCP-1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte chemotactic protein 1; monocyte secretory protein JE; SCYA2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small-inducible cytokine A2; SMC-CF

Rabbit

IgG

C-C motif chemokine 2

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original antibody was generated by phage display using the Human Combinatorial Antibody Library (HuCAL GOLD).

-20°C,2°C to 8°C

12352203