Figure 1. Western blot analysis of CCL3 using anti-CCL3 antibody (PB9022). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat CCL3 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL3 antigen affinity purified polyclonal antibody (Catalog # PB9022) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL3 at approximately 33KD. The expected band size for CCL3 is at 33KD.
Figure 1. Western blot analysis of CCL3 using anti-CCL3 antibody (PB9022). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat CCL3 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL3 antigen affinity purified polyclonal antibody (Catalog # PB9022) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL3 at approximately 33KD. The expected band size for CCL3 is at 33KD.
Anti-CCL3/Macrophage Inflammatory Protein 1 alpha Antibody Picoband(r)
PB9022
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityRat
TargetCcl3
Overview
- SupplierBoster Bio
- Product NameAnti-CCL3 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for CCL3 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA
- Applications SupplierELI, WB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID25542
- Target nameCcl3
- Target descriptionC-C motif chemokine ligand 3
- Target synonymsC-C motif chemokine 3; chemokine (C-C motif) ligand 3; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3); macrophage inflammatory protein 1-alpha; MIP-1a; MIP-1-alpha; Scya3; small-inducible cytokine A3
- HostRabbit
- IsotypeIgG
- Protein IDP50229
- Protein NameC-C motif chemokine 3
- Scientific DescriptionBoster Bio Anti-CCL3/Macrophage Inflammatory Protein 1 alpha Antibody Picoband® catalog # PB9022. Tested in ELISA, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityRat
- Reactivity SupplierRat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203