Anti-CCRL2 [BZ2E3]

Absolute Antibody

Anti-CCRL2 [BZ2E3]

9

This antibody is specific for murine CCRL2 (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, p

This antibody has been used in various FACS analyses, for instance, to confirm that it is only specific to mCCRL2-hemagglutinin (HA)/L1.2 transfectants and that peritoneal macrophages treated with LPS, TNFalpha, IFN-gamma, or poly:IC up-regulate mCCRL2 protein expression (Zabel et al., 2008). Of note, this antibody binding to mouse peritoneal mast cells could be inhibited by chemerin, a protein ligand for signaling receptor CMKLR1 (Zabel et al., 2008). This anti-CCRL2 antibody can be used in wide variety of assays to study leukocytes and immune response. For example, this antibody has been used to examine the expression, regulation, and function of atypical chemerin receptor CCRL2 on endothelial cells (Monnier et al., 2012) and to delineate how the chemoattractant chemerin suppresses melanoma by recruiting natural killer cell antitumor defenses (Pachynski et al., 2012).

Functional Assay, Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry

Research Use Only

Monoclonal

BZ2E3

Ccrl2

chemokine (C-C motif) receptor-like 2

1810047I05Rik; Ack; Ackr5; C-C chemokine receptor-like 2; CCR1; CCR11; chemokine (C-C) receptor 1-like 2; chemokine receptor CCR11; Cmkbr1; Cmkbr1l2; E01; G-protein coupled beta chemokine receptor; L-C; L-CCR; lipopolysaccharide inducible C-C chemokine receptor related; lipopolysaccharide-inducible C-C chemokine receptor; lipopolysacharide-inducible C-C chemokine receptor

Rabbit

IgG

C-C chemokine receptor-like 2

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Mouse

-20°C,2°C to 8°C

12352203