Anti-CCRL2 [BZ5B8]

Absolute Antibody

Anti-CCRL2 [BZ5B8]

7

This antibody is specific for murine CCRL2 (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, p

This antibody has been used in various FACS analyses, for instance, to confirm that it is only specific to mCCRL2-hemagglutinin (HA)/L1.2 transfectants and that peritoneal macrophages treated with LPS, TNFalpha, IFN-gamma, or poly:IC up-regulate mCCRL2 protein expression (Zabel et al., 2008). Of note, this antibody binding to mouse peritoneal mast cells could be inhibited by chemerin, a protein ligand for signaling receptor CMKLR1 (Zabel et al., 2008). This anti-CCRL2 antibody can be used in wide variety of assays to study leukocytes and immune response. For example, this antibody has been used to examine the expression, regulation, and function of atypical chemerin receptor CCRL2 on endothelial cells (Monnier et al., 2012) and to delineate how the chemoattractant chemerin suppresses melanoma by recruiting natural killer cell antitumor defenses (Pachynski et al., 2012).

Functional Assay, Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry

FC; WB; ELISA; IP; IHC; ICC; functional assays

Research Use Only

Monoclonal

BZ5B8

Ccrl2

chemokine (C-C motif) receptor-like 2

1810047I05Rik; Ack; Ackr5; C-C chemokine receptor-like 2; CCR1; CCR11; chemokine (C-C) receptor 1-like 2; chemokine receptor CCR11; Cmkbr1; Cmkbr1l2; E01; G-protein coupled beta chemokine receptor; L-C; L-CCR; lipopolysaccharide inducible C-C chemokine receptor related; lipopolysaccharide-inducible C-C chemokine receptor; lipopolysacharide-inducible C-C chemokine receptor

Rat

IgG2a

C-C chemokine receptor-like 2

Mouse

Mouse

This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide/KLH conjugate. The immunizing amino-terminal mCCRL2 peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1–24 of mCCRL2, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).

-20°C,2°C to 8°C

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