Anti-CD11b [BEAR1]

Absolute Antibody

Anti-CD11b [BEAR1]

9

This antibody is specific for human CB11b. CB11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. CD11b is also identical with CR-3,

To investigate the role of adhesion molecules in the release of histamine, a histamine release assay was performed on human basophils under influence of the mouse version of this antibody (Watanabe et al, 1992; pmid:1384863). To study the expression of complement proteins on the surface of T lymphocytes, flow cytometry was performed on human T cells using the mouse version of this antibody (Wagner et al, 2001; pmid:11298342). To determine whether PMN ADCC also would be increased by the addition of an antibody/GM-CSF fusion protein and whether this would be associated with the up-regulation and activation of Mac-1, flow cytometry was performed on LA-N-1 neuroblastoma cells using the mouse version of this antibody (Metelitsa et al, 2002; pmid:12010822). Flow cytometry was performed on human full blood to create a standardized test for CD11b expression in PMN cells (Latger-Cannard et al, 2004; pmid:15163315). To study the uptake of L. pneumophila in monocytes using complement proteins, flow cytometry was used on MM6 cells using the mouse version of this antibody (Weissgerber et al, 2003; pmid:12620617). Immunofluorescence on isolated PMN was performed using the mouse version of this antibody to understand the action of surface bound elastase on PMN (Zimmermann et al, 2005; pmid:15718918). To study the interaction between CR3 and phagocytosis, an interaction experiment was performed between CD16 and PBMCs using the mouse version of this antibody as a blocking antibody. Furthermore, this antibody was used to immunophenotype PBMCs (Preynat-Seauve et al, 2004; pmid:14971040).

Functional Assay, Flow Cytometry, ImmunoFluorescence, Neutralisation/Blocking

Research Use Only

Monoclonal

BEAR1

ITGAM

integrin subunit alpha M

antigen CD11b (p170); CD11 antigen-like family member B; CD11B; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; macrophage-1 antigen alpha subunit; MO1A; neutrophil adherence receptor alpha-M subunit; SLEB6

Human

IgG1

Integrin alpha-M

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

-20°C,2°C to 8°C

12352203