Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CD11b Antibody(M00144) ITGAM was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGAM Antibody (M00144)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-CD11b Rabbit Monoclonal Antibody
M00144
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-CD11b Rabbit Monoclonal Antibody
- Delivery Days Customer9
- CertificationResearch Use Only
- UNSPSC12352203