Anti-CD127 [1A11 H3L4]

Absolute Antibody

Anti-CD127 [1A11 H3L4]

7

The binding affinity of this antibody for human interleukin 7 receptor was determined using BIACORE analysis and was reported to be Kd= 22 pM. This antibody demonstrated complement dependent cytotoxicity on HEK293 cells expressing hIL-7R in vitro. This antibody showed inhibition of IL-7-induced STAT5 phosphorylation in human polymorphonuclear monocytes and IL-17 production in differentiated human Th17 cells in an in vitro assay (US9150653). The safety, pharmacokinetics, target engagement, pharmacodynamics and immunogenicity of this antibody was studied in healthy subjects. Single doses of this antibody (0.6 mg kg-1 or 2.0 mg kg-1) were well tolerated, increased circulating IL-7 and soluble CD127, and elicited antidrug antibodies with neutralizing capability, but did not have an impact on lymphocyte populations, inflammatory cytokines or differentiation markers. It blocked IL-7 receptor signaling upon full target engagement. This antibody may effectively modulate the autoinflammatory activity of pathogenic T cells in diseased tissue (PMID: 30161291). The characterization of humoral response generated after administration of this antibody was studied in a single-dose escalation phase I study (PMID: 33755713).

ELISA, Neutralisation/Blocking

inhibit; ELISA; immunotherapy

Research Use Only

Monoclonal

1A11 H3L4

IL7R

interleukin 7 receptor

CD127, CDW127, IL-7R-alpha, IL-7Ralpha, IL7RA, IL7Ralpha, ILRA, IMD104, lnc-IL7R, sIL-7R, interleukin-7 receptor subunit alpha, CD127 antigen, IL-7 receptor subunit alpha, IL-7R subunit alpha, interleukin 7 receptor alpha chain, soluble interleukin-7 receptor

Mouse

IgG2b

Interleukin-7 receptor subunit alpha

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Human, Monkey, Primate

Human, cynomolgus monkey, marmoset

The original parental mouse antibody1A11 was generated by immunizing C57BL/6 mice with a synthetic peptide (300 mug) of myelin oligodendrocyte glycoprotein (MOG residues 35-55). This antibody is a humanized and affinity matured version of 1A11.

-20°C,2°C to 8°C

41116161