Anti-CD134 [A10-F]

Absolute Antibody

Anti-CD134 [A10-F]

9

This antibody binds the human OX40, blocks binding of OX40L to OX40 and inhibits one or more functions associated with binding of OX40L to OX40. It also cross reacts with OX40 from cynomolgus and rhesus monkey.

This antagonist antibody is a humanized version of the original murine parent antibody A10. The binding specificity of this antibody to OX40 was determined by ELISA and flow cytometric techniques. Naive CD4+ T-cell assay was used to test the ability to inhibit cytokine production, proliferation, and to induce apoptosis in human CD4+ T-cells. A10-F inhibited cytokine release (IL-2/IL-5/IL-13) and CD4+ T-cells proliferation to the same level as its murine parent. A10-F was also tested for its antagonistic activities like inhibiting the proliferation and cytokine release of Th1 primed T-cells, Th2 primed T-cells and Th17 cells in the presence of irradiated OX40L transfected L-cells or parental L-cells. Under Th1 conditions, A10-F inhibited the production of IL-2, IL-5, IL-13 cytokines and cell proliferation. Under Th2 conditions, A10-F showed an antagonistic effect on activated CD4+ T-cells by inhibiting their proliferation and cytokine release (IL-2, IL-5, and IL-13). With Th17 cells, A10-F increased apoptosis in a dose dependent manner. Cell proliferation and cytokine production in Th2 cells primed with TSLP-activated myeloid CD11c+ dendritic cells (DCs) was inhibited in the presence of A10-F. It also further caused significant increase in apoptosis and a lower expression of Survivin. A10-F was also tested for its antagonistic activities and the ability to inhibit the proliferation and cytokine production of Th1 primed T-cells in the presence of LPS-activated DCs. Here also A10-F inhibited the IL-2, IFN-gamma, and TNF-alpha cytokines production and cell proliferation (US20100136030).

Flow Cytometry, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

A10-F

TNFRSF4

TNF receptor superfamily member 4

ACT35; ACT35 antigen; ATC35 antigen; CD134; CD134 antigen; IMD16; lymphoid activation antigene ACT35; OX40; OX40 antigen; OX40 cell surface antigen; OX40 homologue; OX40L receptor; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; tumor necrosis factor receptor superfamily member 4; TXGP1L

Mouse

IgG2b

Tumor necrosis factor receptor superfamily member 4

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human, Monkey, Primate

-20°C,2°C to 8°C

12352203