Anti-CD153 [RM153]

Absolute Antibody

Anti-CD153 [RM153]

9

This antibody is specific for murine CD153, a 40-kDa type II transmembrane protein member of the TNF superfamily. Interaction of CD30 with CD153 plays an important role in T cell activation/costimulation.

This antibody has been used in various FACS analyses, for instance, to demonstrate that lymphoid tissue inducer-like cells are an innate source of IL-17 and IL-22 (Takatori et al, 2009), and to suggest that CD30 ligand/CD30 plays a critical role in Th17 differentiation in mice (Sun et al, 2010). Importantly, the pharmacological effects of this rat anti-mouse CD153 mAb (clone RM153; IgG2b isotype) have been reported in a number of settings. For example, this antibody has been shown to prevent or delay the spontaneous development of diabetes in non-obese diabetic mice when treatment is initiated in young mice (Chakrabarty et al, 2003), and to prolong the survival of mice in a model of CD4+ T-cell-mediated lethal graft-versus-host disease induced by injection of C57BL/6 cells into MHC class II-only disparate bm12 recipients (Blazar et al, 2004). This antibody has also been used as parts of in vivo functional assays to demonstrate that early CD30 signaling is critical for Treg-mediated acute graft-versus-host disease protection after major MHC-mismatch bone marrow transplantation (Zeiser et al, 2007), and to provide direct evidence that pathogenic memory T cells are amenable to suppression in an antigen-specific manner while identifying CD30 as a molecule that is critical for the regulation of memory T cell responses (Dai et al, 2004).

Functional Assay, Flow Cytometry, Neutralisation/Blocking

Research Use Only

Monoclonal

RM153

Tnfsf8

tumor necrosis factor (ligand) superfamily, member 8

CD153; Cd30; CD30 ligand; Cd30l; CD30-L; CD30LG; Tnlg3a; tumor necrosis factor ligand 3a; tumor necrosis factor ligand superfamily member 8

Mouse

IgG2a

Tumor necrosis factor ligand superfamily member 8

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Mouse

12352203