Anti-CD163 [2A10 (2A10/11)]

Absolute Antibody

Anti-CD163 [2A10 (2A10/11)]

7

This antibody recognizes and binds porcine CD163.

This antibody is recommended to be used as a tool in the study of porcine macrophage maturation and differentiation and for determining their heterogeneity both in normal and pathological conditions. This antibody can recognize porcine CD163 in a western blot assay and an immunoprecipitation assay (PMID: 9151393). 2A10 successfully binds PAM cells in a flow cytometric analysis (PMID:23785195). Porcine CD163 has also been analyzed as potential antigen targeting receptors using mouse IgG as surrogate antigens. MAbs to CD163 induced in vitro PBMC proliferation at concentrations 50-80 fold lower than the control mAb when using, as responder cells, cells from pigs immunized with mouse serum IgGs (PMID:21557980). It is also reported that porcine mAb 2A10 antigen is homologous to human CD163 and related to macrophage differentiation (PMID: 10227997). This antibody was also used to study the role of CD163 in the process of infection of porcine monocytes/macrophages by African swine fever virus infection (PMID: 14648288). This mAb was used in a sandwich enzyme-linked immunoassay (ELISA) to measure levels of sCD163 in porcine sera and biological fluids (Perez et al, 2008).

Flow Cytometry, ImmunoFluorescence, ELISA

FC; IF; ELISA

Research Use Only

Monoclonal

2A10 (2A10/11)

CD163

CD163 molecule

scavenger receptor cysteine-rich type 1 protein M130

Rabbit

IgG

Scavenger receptor cysteine-rich type 1 protein M130

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Porcine

Pig (Sus scrofa)

The original antibody was generated by immunizing BALB/c mice with porcine alveolar macrophages.

-20°C,2°C to 8°C

12352203