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Immunohistochemical staining of formalin fixed and paraffin embedded human spleen tissue section using anti-CD163 rabbit monoclonal antibody (Clone RM371) at a 1:1000 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human spleen tissue section using anti-CD163 rabbit monoclonal antibody (Clone RM371) at a 1:1000 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human spleen tissue section using anti-CD163 rabbit monoclonal antibody (Clone RM371) at a 1:1000 dilution.

anti-CD163 (human), Rabbit Monoclonal (RM371)

REV-31-1257-00
RevMAb Biosciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCD163
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Overview

  • Supplier
    RevMAb Biosciences
  • Product Name
    anti-CD163 (human), Rabbit Monoclonal (RM371)
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RM371
  • Gene ID9332
  • Target name
    CD163
  • Target description
    CD163 molecule
  • Target synonyms
    hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; SCARI1; scavenger receptor cysteine-rich type 1 protein M130
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86VB7
  • Protein Name
    Scavenger receptor cysteine-rich type 1 protein M130
  • Scientific Description
    CD163 (M130 antigen; Ber-Mac3, Ki-M8; SM4) is a membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. - Recombinant Antibody. This antibody reacts to human CD163. Applications: WB, IHC. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. CD163 (M130 antigen; Ber-Mac3, Ki-M8; SM4) is a membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD163 using anti-CD163 antibody (A00812-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD163 antigen affinity purified polyclonal antibody (Catalog # A00812-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD163 at approximately 150 kDa. The expected band size for CD163 is at 128 kDa.
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