Anti-CD171 [L1-14.10]

Absolute Antibody

Anti-CD171 [L1-14.10]

7

This antibody is specific for the ectodomain (amino acids M1 to E1123) of human L1, and recognises an epitope in the third Ig domain. L1 is over-expressed in a range of tumours, including neuroblastomas, renal carcinomas, ovarian and endometrial carcinoma

The specificity of this antibody has been confirmed in flow cytometric (Schafer et al, 2012; Huszar et al, 2006) and Western blot analysis (Huszar et al, 2006; Wolterink et al, 2010). This antibody has been shown, when incubated with SKOV3ip human ovarian carcinoma cells, to efficiently and sustainedly block extracellular signal-regulated kinase (Erk) phosphorylation (Gast et al, 2008). This antibody has been used in immunohistochemical staining for L1 expression, using paraffin-embedded human ovarian carcinoma tissue sections (Wolterink et al, 2010; Knogler et al, 2007) and a panel of human tumour tissue sections, including benign and malignant tumours of the female genital tract (Huszar et al, 2006), and renal cell carcinoma, schwannoma, gastric conventional GIST, pancreatic neuroendocrine tumor and paraganglioma (Inaguma et al, 2016). This antibody, when administered to SCID mice in combination with cytostatic drugs, efficiently reduces the growth of subcutaneously grown Colo357 or SKOV3ip tumors (Schafer et al, 2012).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry

IF; WB; IP; IHC; FC

Research Use Only

Monoclonal

L1-14.10

L1CAM

L1 cell adhesion molecule

antigen identified by monoclonal antibody R1; CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAML1; N-CAM-L1; NCAM-L1; neural cell adhesion molecule L1; S10; SPG1

Rabbit

IgG

Neural cell adhesion molecule L1

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

This antibody was raised by immunising mice with recombinant L1-Fc fusion protein, consisting of the ectodomain of human L1.

-20°C,2°C to 8°C

12352203