Figure 1. Western blot analysis of CD2 using anti-CD2 antibody (PB9929). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse cardiac muscle tissue lysates, Lane 2: HEPA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD2 antigen affinity purified polyclonal antibody (Catalog # PB9929) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD2 at approximately 58 kDa. The expected band size for CD2 is at 38 kDa.
Figure 1. Western blot analysis of CD2 using anti-CD2 antibody (PB9929). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse cardiac muscle tissue lysates, Lane 2: HEPA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD2 antigen affinity purified polyclonal antibody (Catalog # PB9929) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD2 at approximately 58 kDa. The expected band size for CD2 is at 38 kDa.
Anti-CD2/CD2 Antibody Picoband(r)
PB9929
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetCD2
Overview
- SupplierBoster Bio
- Product NameAnti-CD2 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID914
- Target nameCD2
- Target descriptionCD2 molecule
- Target synonymsCD2 antigen (p50), sheep red blood cell receptor; erythrocyte receptor; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; rosette receptor; SRBC; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5
- HostRabbit
- IsotypeIgG
- Protein IDP08920
- Protein NameT-cell surface antigen CD2
- Scientific DescriptionBoster Bio Anti-CD2/CD2 Antibody Picoband® catalog # PB9929. Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203