Anti-CD20 [18B12]

Absolute Antibody

Anti-CD20 [18B12]

7

This antibody is specific for murine CD20, which is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells. Low numbe

In the original publication, this antibody was used in FACS and in vivo functional assays to examine how B cell depletion affects autoimmunity (Ahuja et al., 2007). In other studies, this antibody has been used mainly in FACS, immunofluorescence, and various in vitro and in vivo functional experiments. More recent works employing this antibody have shown, for instance, that enhanced phagocytosis of circulating B cells by Kupffer cells represents an important in vivo mechanism for improved activity of glycoengineered anti-CD20 mAbs (Grandjean et al., 2016), that B lymphocytes limit senescence-driven fibrosis resolution and favor hepatocarcinogenesis in mouse liver injury (Faggioli et al., 2017), and that B cells inhibit the antitumor immunity against an established murine fibrosarcoma (Maglioco et al., 2017).

Functional Assay, Flow Cytometry, ImmunoFluorescence, Western Blot, Other Application

FC; IF; WB; depleting; functional assays

Research Use Only

Monoclonal

18B12

Ms4a1

membrane-spanning 4-domains, subfamily A, member 1

AA960661; B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; Cd20; CD20 antigen; Ly-4; Ly-44; lymphocyte antigen 44; membrane-spanning 4-domains, subfamily A, member 2; Ms4a2

Mouse

IgG2a

B-lymphocyte antigen CD20

This is a bispecific, knob-into-hole heterodimerized antibody, with one arm directed against mouse CD20 (Fab) and one arm directed against mouse CD3e (scFv).

Mouse

Mouse

This antibody was generated by immunizing CD20−/− mice with CD20-transfected cell lines (300.18 and 70Z3) and CD20-peptide-keyhole limpet hemocyanin conjugate.

-20°C,2°C to 8°C

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