Anti-CD20 [NCD1.2 (scFv-3)]

Absolute Antibody

Anti-CD20 [NCD1.2 (scFv-3)]

9

This antibody is specific for the amino acid sequence PSEKNSLSI of dog CD20. It also cross reacts with human CD20. CD20 is a 33kDa tetraspanning transmembrane phosphoprotein which is expressed in pre B-cells. This B-lymphocyte-specific membrane protein pl

The original IgG antibody binds biotinylated canine CD20 peptide with an relative affinity of kd= 340 pM. This antibody was able to recognize endogenous canine CD20 protein in canine 3132 lymphoma cells and also cross reacted with transfected human GFP-CD20 expression construct in H1299 cells in an western blot assay. This antibody can detect canine CD20 expressed on normal peripheral blood, medium-sized B-cell lymphoma and diffuse large B-cell lymphoma in a flow cytometric assay. It could not detect CD20 -negative peripheral T-cell lymphoma cells in flow cytometry. This antibody was also used in the immunohistochemical staining of formalin fixed samples of diffuse large B-cell lymphoma and lymphoma histotypes of B-cell origin, including marginal zone lymphoma, follicular lymphoma, and small lymphocytic lymphoma. (PMID: 26894679)

Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry

Research Use Only

Monoclonal

NCD1.2 (scFv-3)

MS4A1

membrane spanning 4-domains A1

B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; Bp35; CD20; CD20 antigen; CD20 receptor; CVID5; FMC7; LEU-16; leukocyte surface antigen Leu-16; membrane-spanning 4-domains, subfamily A, member 1; S7

Canine

IgG4

B-lymphocyte antigen CD20

This chimeric dog antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Canine, Human

-20°C,2°C to 8°C

12352203