Anti-CD20 [NCD1.2 (scFv-3)]

Absolute Antibody

Anti-CD20 [NCD1.2 (scFv-3)]

9

This antibody is specific for the amino acid sequence PSEKNSLSI of dog CD20. It also cross reacts with human CD20. CD20 is a 33kDa tetraspanning transmembrane phosphoprotein which is expressed in pre B-cells. This B-lymphocyte-specific membrane protein pl

The original IgG antibody binds biotinylated canine CD20 peptide with an relative affinity of kd= 340 pM. This antibody was able to recognize endogenous canine CD20 protein in canine 3132 lymphoma cells and also cross reacted with transfected human GFP-CD20 expression construct in H1299 cells in an western blot assay. This antibody can detect canine CD20 expressed on normal peripheral blood, medium-sized B-cell lymphoma and diffuse large B-cell lymphoma in a flow cytometric assay. It could not detect CD20 -negative peripheral T-cell lymphoma cells in flow cytometry. This antibody was also used in the immunohistochemical staining of formalin fixed samples of diffuse large B-cell lymphoma and lymphoma histotypes of B-cell origin, including marginal zone lymphoma, follicular lymphoma, and small lymphocytic lymphoma. (PMID: 26894679)

Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry

Research Use Only

Monoclonal

NCD1.2 (scFv-3)

MS4A1

membrane spanning 4-domains A1

B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; Bp35; CD20; CD20 antigen; CD20 receptor; CVID5; FMC7; LEU-16; leukocyte surface antigen Leu-16; membrane-spanning 4-domains, subfamily A, member 1; S7

B-lymphocyte antigen CD20

Canine, Human

-20°C,2°C to 8°C

12352203