Anti-CD22 [To15]

Absolute Antibody

Anti-CD22 [To15]

7

This antibody recognises epitope C on the fourth Ig-like domain of the human CD22. CD22 which is a representative of the immunoglobulin superfamiliy and binds sialylated ligands. Its involved in the interactions between B-cell interactions. It is higly ex

IP: To15 antibody was used for the immunoprecipitation of the products of IgM kinase reaction to examine association between CD22 and B-cell receptor (Peaker and Neurberger, 1993); another group was also successfull in using this abtybody for immunoprecipitation (Zhang and Varki, 2004). Furthermore, it was demonstrated that To15 antibody was able to block CD22-mediated adhesion (specifically, the binding of Daudi cells to COS-CD cells) in around 40% of cases, which presents a potential for using this antibody in CD22 inhibition in the immune disease (Tedder et al., 1996). IHC: This antibody was also used to co-stain histological samples of lymph node biopsies in an attempt to immunologically characterise Langerhans cells in lymph nodes (Ralfkiaer et al., 1984).

Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry

IHC; FACS; IP; adhesion assays

Research Use Only

Monoclonal

To15

CD22

CD22 molecule

B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigen; sialic acid-binding Ig-like lectin 2; SIGLEC2; SIGLEC-2; T-cell surface antigen Leu-14

Human

IgG1

B-cell receptor CD22

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

This antobody was raised by immunising Balb-c mice with a potassium iodide extract of normal human tonsil cell membranes and subsequent creation of hybridomas.

-20°C,2°C to 8°C

12352203