Anti-CD22 [To15]

Absolute Antibody

Anti-CD22 [To15]

7

This antibody recognises epitope C on the fourth Ig-like domain of the human CD22. CD22 which is a representative of the immunoglobulin superfamiliy and binds sialylated ligands. Its involved in the interactions between B-cell interactions. It is higly ex

IP: To15 antibody was used for the immunoprecipitation of the products of IgM kinase reaction to examine association between CD22 and B-cell receptor (Peaker and Neurberger, 1993); another group was also successfull in using this abtybody for immunoprecipitation (Zhang and Varki, 2004). Furthermore, it was demonstrated that To15 antibody was able to block CD22-mediated adhesion (specifically, the binding of Daudi cells to COS-CD cells) in around 40% of cases, which presents a potential for using this antibody in CD22 inhibition in the immune disease (Tedder et al., 1996). IHC: This antibody was also used to co-stain histological samples of lymph node biopsies in an attempt to immunologically characterise Langerhans cells in lymph nodes (Ralfkiaer et al., 1984).

Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry

IHC; FACS; IP; adhesion assays

Research Use Only

Monoclonal

To15

CD22

CD22 molecule

B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigen; sialic acid-binding Ig-like lectin 2; SIGLEC2; SIGLEC-2; T-cell surface antigen Leu-14

Mouse

IgG2b

B-cell receptor CD22

Human

Human

This antobody was raised by immunising Balb-c mice with a potassium iodide extract of normal human tonsil cell membranes and subsequent creation of hybridomas.

-20°C,2°C to 8°C

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