Anti-CD25 [32]

Absolute Antibody

Anti-CD25 [32]

9

The antibody specifically binds to chicken CD25, a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also called interleukin-2 receptor alpha chain, and togethe

The original antibody detected the recombinant chicken CD25 by western blot from Con A-stimulated spleen cells. The original antibody detected CHO cells stably transfected with chCD25/IgG4 by flow cytometry. Furthermore, the antibody stained HD11 chicken macrophages, spleen mononuclear cells, and bursa derived lymphocytes. The antibody stained of MDV-transformed T cell lines, UD35 and US53, reticuloendothelial virus-transformed B cell line, CU60, and avianleukosis virus transformed macrophage cell line, HTC. This antibody was used in immunofluorescence in different tissues (spleen, bursa of Fabricius, and intestinal duodenum). The antibody blocked IL-2-dependent spleen cell proliferation and nitric oxide production (Lee et al, 2011; pmid: 21872344).

Flow Cytometry, ImmunoFluorescence, Western Blot

Research Use Only

Monoclonal

32

Mouse

IgM

Chicken

-20°C,2°C to 8°C

12352203