Anti-CD25 [7D4]

Absolute Antibody

Anti-CD25 [7D4]

7

7D4 recognizes mouse CD25 at an epitope comprising amino acid 184 to 194 (REHHRFLASEE). This epitope is distinct from the IL-2 binding site and does not block binding of IL-2 to CD25. Murine CD25 is a 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha

7D4 is a non-IL-2 blocking anti-CD25 antibody that has been widely used to identify CD25-positive cells via flow cytometry (Kohyama et al., 2012; pmid: 22399592), for instance, to analyse Lymph node cells from BALB/c mice that were untreated or treated with 0.25 mg PC61 (Onizuka et al., 1999; pmid: 10397255) or to characterize brain-infiltrating immune cells (Maes et al., 2013). Although 7D4 does not inhibit IL-2 binding itself, it shows synergistic qualities with a cancer vaccine; namely, 7D4 with a cancer vaccine boosted the vaccine-induced anti-tumour response. Furthermore, this antibody was shown to be able to induce inactivation of regulatory T cells (Treg); the effect was particularly significant when this clone was used synergistically with another anti-CD25 clone - PC61 (Ab01107) (Kohm et al., 2006; pmid: 16517695; McNeill et al., 2007; pmid: 17212768). 7D4, when turned into a mouse IgG2a format, exhibits depleting qualities against Treg cells and can be used to remove Treg lymphocytes from the tumoral environment without blocking CD25 (IL-2 pathway) on T effector cells (Solomon et al., 2020). As a result, it can be utilized as a potential immunotherapy agent achieving Treg depletion without T effector cells debilitation (Solomon et al., 2020). Finally, it was demonstrated that such anti-CD25 non-IL-2 blocking antibodies are successful agents in promoting effector activation and leading to strong antitumor activity (Solomon et al., 2020).

Flow Cytometry, Other Application

FC; depleting

Research Use Only

Monoclonal

7D4

Il2ra

interleukin 2 receptor, alpha chain

CD25; I; IL-2 receptor subunit alpha; Il2r; IL-2R alpha chain; IL-2R subunit alpha; IL-2-RA; IL2-RA; interleukin-2 receptor subunit alpha; Ly-4; Ly-43; p55 chain

Rat

IgG1

Interleukin-2 receptor subunit alpha

This reformatted rat antibody was made using the variable domain sequences of the original rat IgM format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

The original rat IgM version of this antibody was raised by immunizing Lewis rats with HT2 cells in complete Freund's adjuvant via a intraperitoneal injection and subsequent generation of hybridomas.

-20°C,2°C to 8°C

12352203