Anti-CD27 [LG.3A10]

Absolute Antibody

Anti-CD27 [LG.3A10]

7

This antibody is specific for mouse CD27, which, as in the human system, is largely restricted to cells of the T cell lineage, and is expressed on the great majority of both alphabeta and gammadelta T lymphocytes, and thymocytes.

This antibody is specific for murine CD27, and has been used to assess the distribution of CD27 expression using IF, FC and IP analysis (Gravstein, 1995). Upon cross-linking, this antibody has been shown to induce a 4-fold increase in T cell proliferation in response to suboptimal stimulation with concanavalin A (Gravestein, 1995), indicating that this antibody is able to mimic ligand binding.

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Other Application

Activating; IP; IF; FC

Research Use Only

Monoclonal

LG.3A10

Cd27

CD27 antigen

CD antigen 27; CD27 antigen; CD27L receptor; S15; S152; T-cell activation antigen CD27; Tnfr; Tnfrsf7; Tp5; Tp55; tumor necrosis factor receptor superfamily member 7

Mouse

IgG2a

CD27 antigen

This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Human, Mouse, Rat

Mouse, Human, Rat

This antibody was produced by injecting Armenian hamsters intraperitoneally with a mixture of two murine CD27 tag-expressing Armenian hamster fibroblast clones (AR0524 and AR0530) in Freund's adjuvant

-20°C,2°C to 8°C

12352203