Anti-CD32 [AT130-5], Mouse IgG1-D265A, Fc Silenced, kappa

Absolute Antibody

Anti-CD32 [AT130-5], Mouse IgG1-D265A, Fc Silenced, kappa

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This antibody is specific for murine CD32, an Fc gamma surface receptor protein with a low affinity for IgG antibodies that expresses on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. CD32 binds antibody-antigen i

The original version of this antibody (mouse IgG1) was used in various FACS, Western blot, and immunofluorescence experiments to demonstrate that, for example, interaction with FcgammaRIIB is critical for the agonistic activity of anti-CD40 mAb (White et al., 2011; PMID: 21742972), and that immunotherapy targeting murine FcgammaRIIB is limited by mAb consumption and receptor internalization (Williams et al., 2013; PMID: 24026082). It is noted that, unlike the antagonistic anti-CD32 (clone AT128) antibody, which blocks FcgammaRIIB receptor phosphorylation, this agonistic anti-CD32 (clone AT130-5) has been shown to activate receptor function while blocking immune complex engagement to the inhibitory FcgammaRs (Vollack et al., 2017; PMID: 28492697). These differential antagonistic/agonistic anti-CD32 mAbs have been used to probe more specifically the role of CD32 in the murine FVIII-specific recall response (Vollack et al., 2017; PMID: 28492697).

Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

AT130-5

Fcgr2b

Fc receptor, IgG, low affinity IIb

AI528646; CD32; F630109E10Rik; fc gamma receptor IIB; Fc gamma RIIB; Fc[g]; Fc[g]RII; Fcg; Fcgamm; fc-gamma RII; Fc-gamma-RII; FcgammaRIIB; fc-gamma-RIIB; Fcgr; Fcgr2; Fcgr2a; FcgRII; Fcr-; Fcr-2; Fcr-3; fcRII; igG Fc receptor II beta; low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; Ly-1; Ly-17; LyM-; LyM-1; Ly-m2; Ly-m20; lymphocyte antigen 17

Mouse

IgG1

Low affinity immunoglobulin gamma Fc region receptor II

Mouse

-20°C,2°C to 8°C

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