Anti-CD32 [IV.3]

Absolute Antibody

Anti-CD32 [IV.3]

7

This antibody recognizes and binds CD32a.

This antibody successfully precipitated a 40-kd FcR from lysates of U937 and K562 cells but not from Daudi or Raji cells. It selectively inhibited the binding of murine IgG1-coated erythrocytes to U937 cells. IV.3 blocked mIgG1 anti-T3 (Leu-4) stimulation but not mIgG2a anti-T3 (OKT3) stimulation of T cells (PMID: 2936805). Anti-FcyRII antibody IV.3 specifically stained Ltk- cells transfected with the FcyR cDNA clone in an immunofluorescence assay (PMID: 2824655). It was reported that IV.3 induces anaphylaxis in immunocompetent CD32a-transgenic FCGR2A mice and that IV.3 also induce severe thrombocytopenia in FCGR2A mice (PMID: 26396093). IV.3, reacted with CD32A-R131 exclusively, confirming the specificity in an ELISA (PMID:17386079).

ImmunoFluorescence, ImmunoPrecipitation, ELISA, Neutralisation/Blocking

IP; Inhibit; IF; Block; ELISA

Research Use Only

Monoclonal

IV.3

FCGR2A

Fc fragment of IgG receptor IIa

CD32; CD32A; CDw32; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc gamma receptor RIIa3; FCG2; fc-gamma-RIIa; FcGR; FCGR2; FCGR2A1; fcRII-a; IGFR2; igG Fc receptor II-a; Immunoglobulin G Fc receptor II; low affinity immunoglobulin gamma Fc region receptor II-a

Mouse

IgG2b

Low affinity immunoglobulin gamma Fc region receptor II-a

Human

Human

The original antibody was generated against the FcR of K562 cells.

-20°C,2°C to 8°C

12352203