Anti-CD32b [NOV0281], Human IgG1-STR, STR Fc silenced, lambda

Absolute Antibody

Anti-CD32b [NOV0281], Human IgG1-STR, STR Fc silenced, lambda

9

The antibody specifically binds to CD32b. The antibody mainly recognizes epitope I (i.e., the Fc binding domain area). The antibody does not cross reacts with human CD32a-R.

The specificity of the antibody for CD32b was determined by ELISA analysis. The antibody was able to bind to CD32b expressed on human B cells by flow cytometry analysis (binding affinity 5.4 nM). The binding of the antibody to the BJAB cell line was evaluated by flow cytometry (binding affinity 2.4 nM). The antibody bound to CHO cells expressing huCD32b in flow cytometry analysis. The human IgG1 format of the antibody was evaluated for its activity in a primary NK cell based antibody-dependent cell-mediated cytotoxicity (ADCC) assay. The antibody demonstrated concentration dependent specific cell lysis of both cancer cell lines evaluated (Jeko-1 and Karpas422 Cancer Cell) (US20170198040A1).

Flow Cytometry, ELISA, Other Application

Research Use Only

Monoclonal

NOV0281

FCGR2B

Fc fragment of IgG receptor IIb

CD32; CD32B; CDw32; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma receptor IIb; Fc gamma RIIb; FCG2; fc-gamma RII-b; Fc-gamma RII-c; fc-gamma-RIIb; Fc-gamma-RIIc; FCGR2; FCGR2C; fcRII-b; FcRII-c; IGFR2; igG Fc receptor II-b; IgG Fc receptor II-c; low affinity immunoglobulin gamma Fc region receptor II-b; Low affinity immunoglobulin gamma Fc region receptor II-c

Human

IgG1

Low affinity immunoglobulin gamma Fc region receptor II-b

This format contains the STR complete Fc Silencing mutations from mAbsolve. This is a reformatted human IgG1 STR Fc silenced antibody, based on the original human Fab format, created for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203