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ProductsBack/Anti-CD34 [2E 10]

Anti-CD34 [2E 10]

Ab00981-10.3
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
TargetCD34
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD34 [2E 10]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody, labelled with fluoroscein isothiocyanate, has been used in flow cytometry to assess the loss of expression of CD34 epitopes following enzyme incubation (Qian et al., 2008). The antibody has been used as a primary antibody against CD34 in Western blot analysis, using HRP-conjugated goat anti-mouse IgG as a secondary antibody.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Applications Supplier
    FC; IF; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2E 10
  • Gene ID947
  • Target name
    CD34
  • Target description
    CD34 molecule
  • Target synonyms
    CD34 antigen; hematopoietic progenitor cell antigen CD34
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP28906
  • Protein Name
    Hematopoietic progenitor cell antigen CD34
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The antibody was raised by repeatedly immunising BALB/c mice with KG-1a cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD34 using anti-CD34 antibody (PB9053). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (Catalog # PB9053) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD34 at approximately 105KD. The expected band size for CD34 is at 105KD.
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