Anti-CD3e [898H2-6-15]

Absolute Antibody

Anti-CD3e [898H2-6-15]

7

Hybridoma 898H2-6-15 was shown via double-colour flow cytometry analysis to bind swine lymphocyte populations specifically. It was further shown using FACS that purified 898H2-6-15 bound an epitope on CD3e shared by another anti-pig CD3 Ab (STH164). CD3,

The initial identification of 898H2-6-15 showed that it recognised the T-cell receptor-associated glycoprotein CD3, in particular the e chain (Huang et al, 1999). More recently, the 898H2-6-15 VH and VL domains were reformatted into a scFv and conjugated to diphtheria toxin (Wang et al, 2011). This version of the antibody as an immunotoxin has been shown to deplete circulating T cells in swine without observation of any clinical toxicities.

Flow Cytometry, ImmunoHistoChemistry

FC; IHC

Research Use Only

Monoclonal

898H2-6-15

CD3E

CD3e molecule

CD3; CD3 epsilon subunit; T-cell surface glycoprotein CD3 epsilon chain

Mouse

IgG2a

T-cell surface glycoprotein CD3 epsilon chain

Porcine

Pig

Murine antibodies were produced by immunising C3H/HeJ mice with swine peripheral blood lymphocytes. Hybridomas were generated by obtaining splenocytes from the immunised mice and fusing these with the non-Ig-producing myeloma cell line P3X63Ag8.653 via established methods.

-20°C,2°C to 8°C

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