Anti-CD3e [Visilizumab (HuM291)]

Absolute Antibody

Anti-CD3e [Visilizumab (HuM291)]

9

This antibody recognises the human CD3 epsilon. This antibody also competes with OKT3 (Muromonab) for binding site on the human T cells suggesting that they bind overlapping or closely spaced epitopes.

This antibody is derived from its a murine parent antibody called M291. HuM291 was significantly less mitogenic to T cells in vitro as compared to OKT3 and induced the release of much lower levels of the cytokines tumor necrosis factor-alpha, interferon-gamma, and interleukin-10. HuM291 still retained the ability to modulate the CD3 complex and inhibit the mixed lymphocyte reaction. This antibody competes with OKT3 for binding to human T cells, which was detected using flow cytometry (PMID: 10480417). In vitro studies using chimpanzee peripheral blood mononuclear cells (PBMCs), HuM291 was reported to be less mitogenic than OKT3 and effectively depleted peripheral T cells without eliciting clinical signs of cytokine release syndrome, and recovered T cells were functionally normal (PMID: 10480415). Phase 1 trial of HuM291 in patients receiving renal allografts suggested that it was well tolerated and rapidly depleted circulating T cells in a dose-dependent manner and was associated with only mild to moderate symptoms of cytokine release (PMID: 11152101). Treatment with visilizumab (HuM291) in severe steroid-refractory ulcerative colitis induced symptomatic response and clinical response (PMID: 19714757). The (99m)Tc labeled version of this antibody is also recommended for imaging of human T cells and study of human T-lymphocyte trafficking and lymphocytic infiltration of tissues and organs (PMID: 19759100). This antibody is also reported to have some activity in advanced glucocorticoid-refractory acute graft-versus-host disease (GVHD) (PMID: 11929757).

Flow Cytometry

Research Use Only

Monoclonal

Visilizumab (HuM291)

CD3E

CD3e molecule

CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; IMD18; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

Mouse

IgG2b

T-cell surface glycoprotein CD3 epsilon chain

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human, Primate

-20°C,2°C to 8°C

12352203