Anti-CD4 [5A8]

Absolute Antibody

Anti-CD4 [5A8]

7

This antibody is specific for nonreduced CD4. Human V1 residues (through amino acid 83) are not required for efficient 5A8 binding. human residues of the rod-like B strand which connects V1 and V2 (AA83 -AA105) significantly influence 5A8 binding. human C

This antibody was used for a radioimmunoassay on human rsCD4 (US5871732). It was tested whether this antibody can block HIV-induced syncytia formation (US5871732) (Burkly et al, 1992; pmid:1380539). Western blot was used on different epitopes of CD4 using this antibody (US5871732). Immunohistochemistry was performed on human tissue with a humanized version of this antibody (Boon et al, 2002; pmid:11893418). ELISA with this antibody was used to detect bound sCD4 (Moore et al, 1992; pmid:1378510).

Western Blot, ELISA, ImmunoHistoChemistry, RadioImmunoAssay

RIA; WB; ELISA; IHC

Research Use Only

Monoclonal

5A8

CD4

CD4 molecule

CD4 antigen (p55); CD4 receptor; CD4mut; IMD79; OKT4D; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Mouse

IgG1

T-cell surface glycoprotein CD4

Human

Human

The original antibody was generated by immunizing Balb/c mice with CHO cells expresssing full-length human CD4 on the cell surface.

-20°C,2°C to 8°C

12352203