Anti-CD4 [OX-68]

Absolute Antibody

Anti-CD4 [OX-68]

7

This antibody is reactive with rat CD4 domain 3 & 4, and is partly competitive with OX-67 & 69.

This antibody binds to rat CD4. Upon challenge with HIV-1, this antibody was able to significantly inhibit viral entry into HeLa cells expressing a mutant of rat CD4 that binds gp120 (SImon, 1997). Similarly, pre-binding of gp120 resulted in a 50% reduction in binding of this antibody, indicating epitope masking. Analogous effects on viral entry and synctium formation are observed with MAbs against human CD4.

Flow Cytometry, ImmunoPrecipitation, Western Blot, Other Application

FC; WB; IP; SPR; binding assay

Research Use Only

Monoclonal

OX-68

Cd4

Cd4 molecule

CD4 antigen; p55; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; W3/25; W3/25 antigen

Rat

IgG2b

T-cell surface glycoprotein CD4

This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Rat

Rat

This antibody was prepared by subcutaneously injecting BALB/c mice with a soluble form of rat CD4, consisting of domains 3 and 4. A boost of a mixture of free sCD4 and sCD4 bound to cells was administered intravenously.

-20°C,2°C to 8°C

12352203