Anti-CD40 [2E4E4]

Absolute Antibody

Anti-CD40 [2E4E4]

7

2E4E4 antibody is recommended as an effective adjuvant in the development of vaccines as it was demonstrated to have strong agonistic activity against CD40 evoking 25-fold or greater proliferation of peripheral blood mononuclear cells (PBMCs) from livestock species relative to cells incubated with an isotype control (Martin et al., 2017; PMID: 28107431). What is more, it led to a strong nitric oxide release from bovine macrophages, enhanced expression of MHC-II by PBMCs and induced significant expression of IL-1alpha, IL6, IL-8, and TNF-alpha by PBMCs (Martin et al., 2017; PMID: 28107431). Furthermore, this antibody was successfully utilised in the detection of bovine, caprine, ovine and swine CD40 via flow cytometry and immunohistochemistry in the spleen tissue samples (Martin et al., 2017; PMID: 28107431).

Flow Cytometry, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application

agonist; immune activation; IHC; ICC; FC

Research Use Only

Monoclonal

2E4E4

Mouse

IgG1

Goat, Pig, Cow, Sheep

This antibody was raised by immunising BALB/c mice with recombinant extracellular domain of swine CD40 in RIBI adjuvant.

-20°C,2°C to 8°C

12352203