Anti-CD40 [5D12]

Absolute Antibody

Anti-CD40 [5D12]

9

ch5D12 binds specifically to recombinant and native human CD40 ectodomain and has been shown to also bind CD40 in cynomolgus and marmoset monkeys. The antibody requires residues within D1 and D1/B2 for binding (Hager et al, 2003) (D1 domain is crucial for

ch5D12 is an antagonist of the CD40-CD40L pathway. ch5D12 binds to B cells and monocytes, but not to peripheral blood CD4+ and CD8+ cells. ch5D12 was tested in vivo using a marmoset EAE (experimental autoimmune encephalomyelitis) model (recombinant myelin oligodendrocyte glycoprotein (MOG) was used to induce EAE) - model for human multiple sclerosis. ch5D12 was able to prevent development of the symptoms of the disease during the treatment period, reduce the anti-MOG antibody response and reduce the formation of inflammatory lesions in the CNS. ch5D12 binding and specificity can be detected and measured by FC, SPR, ELISA and IP, and CD40+ cells can be stained by the antibody in IHC.

Flow Cytometry, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

5D12

CD40

CD40 molecule

B cell surface antigen CD40; B cell-associated molecule; Bp50; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDW40; p50; TNFRSF5; tumor necrosis factor receptor superfamily member 5

Human

Tumor necrosis factor receptor superfamily member 5

This reformatted human antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.

Human, Monkey, Primate

-20°C,2°C to 8°C

12352203