Anti-CD40 [LOB7/6]

Absolute Antibody

Anti-CD40 [LOB7/6]

9

This antibody is specific for human CD40, a costimulatory protein on the surface of antigen presenting cells. This antibody has been shown to be cross-reactive with canine CD40.

This antibody was used for immunohistochemistry (IHC) of post-mortem brain tissues. In all tissues examined, the blood vessels were stained positively for CD40 (Togo et al., 2000; PMID: 11121537). This antibody was used for flow cytometry (FC) of endothelial cell lines (Lidington et al., 1999; PMID: 10638837), dendritic cells (Urban et al., 1999; PMID: 10403251), and peripheral blood B cells and monocytes (Fan et al., 2012; PMID: 22459705). This antibody was found to be the optimal antibody for CD40 marking in an FC assay of the antibodies tested (LOB7/6, 82105, 11E9, and a rabbit polyclonal antibody). Furthermore, the effect of its binding to CD40 on keratinocyte proliferation and apoptotic rate was investigated; it was found not to inhibit keratinocyte proliferation. Rather, it had the opposite effect, as it enabled the keratinocytes to retain their proliferative potential and did not induce apoptosis (Villarroel Dorrego et al., 2006; PMID: 16487107). This antibodys cross-reactivity with canine CD40 was evaluated, and it was found to be cross-reactive with canine CD40 and to be suitable for FC and immunoprecipitation (IP) (Wang et al., 2007; PMID: 17695590). As shown via SPR and FACS, this antibodys binding to CD40 overlapped with the following: antibodies 3.1.1, 21.2.1, 22.1.1, 23.5.1, 23.29.1 (classified as one group) and 23.25.1, 23.28.1, 24.2.1 (classified as another group) and the commercially available CD40L (US7288251B2).

Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, Other Application

Research Use Only

Monoclonal

LOB7/6

CD40

CD40 molecule

B cell surface antigen CD40; B cell-associated molecule; Bp50; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDW40; p50; TNFRSF5; tumor necrosis factor receptor superfamily member 5

Mouse

IgG2a

Tumor necrosis factor receptor superfamily member 5

Canine, Human

-20°C,2°C to 8°C

12352203