Figure 2. IHC analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10052). CD40/TNFRSF5 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-CD40/TNFRSF5 Antibody (PB10052) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10052). CD40/TNFRSF5 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-CD40/TNFRSF5 Antibody (PB10052) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-CD40 Antibody Picoband(r)
PB10052
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCd40
Overview
- SupplierBoster Bio
- Product NameAnti-CD40/TNFRSF5 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID21939
- Target nameCd40
- Target descriptionCD40 antigen
- Target synonymsAI326936; B-cell surface antigen CD40; Bp5; Bp50; CD40L receptor; GP39; HIGM1; IGM; IMD3; p5; p50; T-BAM; T-cell differentiation antigen; Tnfr; Tnfrsf5; TRAP; tumor necrosis factor receptor superfamily member 5
- HostRabbit
- IsotypeIgG
- Protein IDP27512
- Protein NameTumor necrosis factor receptor superfamily member 5
- Scientific DescriptionBoster Bio Anti-CD40 Antibody Picoband® catalog # PB10052. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203