Anti-CD44 [F8:33]

Absolute Antibody

Anti-CD44 [F8:33]

7

This antibody is specific for CD44vRA, which is a CD44 derived from rheumatoid arthritis patients. CD44vRA contains an extra alanine. At higher concentration it will also bind to other isoforms of CD44. CD44 is cell-surface receptor that plays a role in c

To test the ability of the mouse version of this antibody to bind to CD44vRA, flow cytometry was preformed on Namalwa cells expressing different isoforms of CD44 (Golan et al, 2007; pmid:17383158). Further, it was used in ELISA preformed on different isotypes of the CD44 protein to assess the ability of this antibody to bind specific types of CD44; CD44vRA (CD44 from rheumatoid arthritis patients) was bound most strongly (Golan et al, 2007; pmid:17383158). Furthermore, flowcytometry was preformed to analyse apoptosis of synovial fluid cells. The mouse version of this antibody was used to induced apoptosis (Golan et al, 2007; pmid:17383158). It was administred to arthritic mice to reduce joint inflammation and induced apoptosis above the spontaneous level in the spleen of the mice (Golan et al, 2007; pmid:17383158). Transwell migration assay was preformed to assess the specificity of this antibody. It prevented namalwa-CD44vRA cells from crossing but did not prevent namalwa cells transfected with other forms of CD44 from crossing (US2011207678). Finally, flow cytometry was preformed with this antibody on synovial fluid cells and PBLs. The antibodies bound to the synovial fluid cells but not the PBLs from the same patient (Golan et al, 2007; pmid:17383158).

Functional Assay, Flow Cytometry, ELISA

ELISA; FC; therapeutic; functional assay

Research Use Only

Monoclonal

F8:33

CD44

CD44 molecule (Indian blood group)

CD44 antigen; CDW44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCELL; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronate receptor; IN; Indian blood group antigen; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; phagocytic glycoprotein 1; soluble CD44

Human

IgG1

CD44 antigen

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original mouse antibody was raised by immunizing C57BL/6 mice with soluble CD44vRA. CD44vRA is a form of CD44, containing an extra alanine, derived from rheumatoid arthritis patients.

-20°C,2°C to 8°C

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