Anti-CD44 [Hermes-1]

Absolute Antibody

Anti-CD44 [Hermes-1]

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This antibody is specific for CD44 (gp90 epitope cluster I), which is selectively expressed on normal or transformed lymphoid cells that are able to recognize and bind to high endothelial venules (specialized vessels that mediate lymphocyte exit from the

This antibody binds CD44, so can be used to label CD44+ cells in flow cytometry analysis. As CD44 molecules are used to bind to high endothelial venules (HEV) at the site of lymphoctye entry to lymphoid organs, this antibody can be used to specifically stain HEV-binding cell lines (Jalkanen, 1986). In addition, this antibody blocks the adhesion of the haematopoietic progenitor cell line KG1 to hyaluronic acid (Bendall, 2003), but does not block lymphocyte binding to HEV (Picker, 1989). 7H9D6 partially blocks the binding of this antibody.

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking

Blocking; ELISA; FC; ICC/IF; IHC; IP; Neut; WB

Research Use Only

Monoclonal

Hermes-1

CD44

CD44 molecule (Indian blood group)

CD44 antigen; CDW44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCELL; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronate receptor; IN; Indian blood group antigen; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; phagocytic glycoprotein 1; soluble CD44

Human

CD44 antigen

This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Bovine, Goat, Human, Mouse, Primate, Rabbit, Rat, Sheep

Human, Mouse, Rat, Goat, Primate, Rabbit, Sheep, Bovine

This antibody was produced by immunizing a Fischer rat with human tonsil lymphocytes (10^7cells intraperitoneally in Freund's complete adjuvant). A boost was given 2 weeks later (intravascularly in saline).

-20°C,2°C to 8°C

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