Anti-CD47 [A4]

Absolute Antibody

Anti-CD47 [A4]

9

This nanobody is specific for murine CD47, a glycosylated five transmembrane protein with a small cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with beta3 integr

This nanobody has been shown to bind with high affinity and specificity to mouse CD47, as characterized by SPR and FACS (Sockolosky et al 2016). In vitro functional assays suggest that this nanobody potently blocks SIRPalpha binding to tumor cell-surface CD47, while broadly recognizing mouse hematopoietic and red blood cells without causing erythropenia (Sockolosky et al 2016). The original nanobody was reported to bind immobilized mouse CD47 with a binding affinity of Kd= ~12 pM (Sockolosky et al 2016). Consistent with its species-specific binding properties, this nanobody has been found not to influence human macrophage phagocytosis of human tumor cells. Furthermore, this nanobody has been used to investigate the biological consequences of antagonizing CD47 in syngeneic mouse disease models (Sockolosky et al 2016).

Functional Assay, Flow Cytometry, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

A4

Cd47

CD47 antigen (Rh-related antigen, integrin-associated signal transducer)

9130415E20Rik; AA407862; AI848868; AW108519; B430305P08Rik; IAP; integrin-associated protein; It; Itgp; leukocyte surface antigen CD47; Rh-related antigen

Mouse

IgG1

Leukocyte surface antigen CD47

Mouse

-20°C,2°C to 8°C

12352203