Figure 3. Flow Cytometry analysis of human PBMC cells using anti-CD47 antibody (A00360-2). Overlay histogram showing human PBMC cells stained with A00360-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD47 Antibody (A00360-2, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 3. Flow Cytometry analysis of human PBMC cells using anti-CD47 antibody (A00360-2). Overlay histogram showing human PBMC cells stained with A00360-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD47 Antibody (A00360-2, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Anti-CD47 Antibody Picoband(r)
A00360-2-10UG
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCD47
Overview
- SupplierBoster Bio
- Product NameAnti-CD47 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID961
- Target nameCD47
- Target descriptionCD47 molecule
- Target synonymsantigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; integrin-associated signal transducer; leukocyte surface antigen CD47; MER6; OA3; Rh-related antigen
- HostRabbit
- IsotypeIgG
- Protein IDQ08722
- Protein NameLeukocyte surface antigen CD47
- Scientific DescriptionBoster Bio Anti-CD47 Antibody Picoband® catalog # A00360-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203