Anti-CD47 [B6H12]

Absolute Antibody

Anti-CD47 [B6H12]

9

This antibody binds the extracellular domain of the human CD47, which is broadly distributed on normal adult tissues, as well as ovarian tumors, and is especially abundant in some epithelia and the brain. IAP or integrin assocaited protein has a role in b

This antibody reacts with placental RGD-binding proteins and human neutrophils in an ELISA (PMID: 2785522). This antibody binds CD47 expressed on human neutrophils in a flow cytometric assay. This antibody immunoprecipitated vitronectin receptor (VnR) from detergent-solubilized placental membrane preparations (PMID: 2785522). It is a function-blocking antibody that inhibits CD47 interactions with TSP1, SIRPalpha, some integrins, and EGF receptor (PMID: 19636412; 10572074; 19004835; 26840086). This antibody directly induces killing of tumor cells by NK cells, B cell migratiom, and regulatory T cell differentiation (PMID: 18497546; 10891456; 16951312). B6H12 enhanced antibody-dependent cellular phagocytosis (ADCP) of human acute myeloid leukemia (AML) cells in vitro by human and mouse macrophages (PMID: 19632179). THis antibody also inhibited AML tumor growth in NOD/SCID/IL2Rgamma- (NSG) mice by blocking this dont eat me signal (PMID: 19632179). B6H12 was used in several preclinical studies to explore combining CD47 blockade with therapeutic antibodies that target specific cancers. This antibody in combination with CD20 antibody rituximab, to promote phagocytosis in vitro and eradicated non-Hodgkin lymphoma in NSG mice (PMID: 20813259).

Flow Cytometry, ImmunoPrecipitation, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

B6H12

CD47

CD47 molecule

antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; integrin-associated signal transducer; leukocyte surface antigen CD47; MER6; OA3; Rh-related antigen

Human

IgG1

Leukocyte surface antigen CD47

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

-20°C,2°C to 8°C

12352203